Determination of anthraquinone components in Shaotangshang Gel by HPLC
- VernacularTitle:HPLC测定烧烫伤凝胶中蒽醌类成分的含量
- Author:
Yanping WANG
;
Xinan WU
- Publication Type:Journal Article
- Keywords:
Shaotangshang Gel;
emodin;
chrysophanol;
rhein;
physcion;
aloe-emodin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method of determining five anthraquinone components in Shaotangshang Gel(Rhizoma Coptidis,Cortex Phellodendri Chinensis,Radix et Rhizoma Rhei,etc.). METHODS:HPLC was used to determine emodin,chrysophanol,rhein,physcion,alone-emodin in Shaotangshang Gel.The separation was performed on Yilited-C_(18) Column with MeOH-phosphate acid(0.1%)(80∶20) as the modile phase,the flow rate was 1.0 mL/min and detection wavelength was set at 430 nm. RESULTS: The resolution and the linearity of the method were good with the average recoveries and RSD values of emoldin,chrysophanol,rhein,physcion,aloe-(emodin) of(98.0%),1.3%;97.1%,1.8%;97.8%,1.5%;97.4%,1.7%;96.7%,1.2% respectively.CONCLUSION: This method is sensitive,accurate and can be used for the quality control of Shaotangshang Gel.