Determination of genpioside,baicalin and berberine hydrochloride in Gonglao Quhuo Tablets simultaneously by RP-HPLC
- VernacularTitle:RP-HPLC同时测定功劳去火片中栀子苷、黄芩苷及盐酸小檗碱的含量
- Author:
Yangrong LI
;
Ye JIANG
;
Fu HAO
;
Xiangyu DING
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Gonglao Quhuo Tablets;
genpioside;
baicalin;
berberine hydrochloride
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method of determining genpioside,baicalin and berberine in Gonglao Quhuo Tablets(Caulis Mahoniae,Cortex Phellodendri Chinensis,Radix Scutellariae,etc). METHODS: The mobile phase composed of A acetonitrile,B acetonitrile and 0.5% triethylamine(Ph3.1)(10∶90).The flow rate was 1.0 mL/min with the wavelength at 254 nm. RESULTS:The calibration curves were linear in the ranges of 3.22-206 ?g/mL(r=0.999 9) for genpioside,2.95-186 ?g/mL(r=0.999 9) for baicalin and 1.64-104 ?g/mL(r=0.999 9) for berberine hydrochloride,respectively.The average recoveries were not less than 98.4%. CONCLUSION: This HPLC method is convenient,rapid,accurate and could be used for quality control of production of Gonglao Quhuo Tablets.
