Determining metabolite diosgenin of Ophiopogon japonicus saponin in vivo by HPLC-MS
- VernacularTitle:HPLC-MS监测麦冬皂苷代谢产物薯蓣皂苷元体内过程研究
- Author:
Lan SHEN
;
Yi FENG
;
Desheng XU
;
Yumin LIU
;
Yijian LAI
- Publication Type:Journal Article
- Keywords:
HPLC-MS;
solid phase extraction(SPE);
diosgenin
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the low content of Ophiopogon japonicus saponin metabolite in vivo. METHODS: HPLC-MS method of determining rat's metabolite diosgenin in vivo was established after single-dose oral Ophiopogon japonicus saponin enteric microsphere. RESULTS: The detection limit was about 50 ng/mL,Linearity,precision of intra and inter-day and reproducibility of the method were good. CONCLUSION: This method accords with the analysis requirement.It can give an effective measure and foundation for studying TCM saponin bioavailability.
