Effects of dexamethasone on toll-like receptor(TLR)2 and 4 in CD14+ monocyte of patients during and after cardiac surgery with cardiopulmonary bypass
- VernacularTitle:体外循环时糖皮质激素对CD14~+单核细胞表面TLR2、4表达的影响
- Author:
Delin ZHANG
;
Yun ZHAO
;
Shengmei ZHU
- Publication Type:Journal Article
- Keywords:
Dexamethason Heat-shock proteins 70 Extracorporeal circulation Antigens,CD14 Toll-like receptors
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the kinetics processes of TLR2 and TLR4 in CD14~+ monocyte of patients during and after cardiac surgery with cardiopulmonary bypass(CPB) and the effects of dexamethasone(DXM) on the regulation of TLR2 and 4 in CD14~+ monocyte. Methods Twenty patients undergoing elective atrial/ventricular septal defect correction were randomized to received 1 mg/kg dexamethasone or placebo before induction of anesthesia. The CD14~+ monocyte surface TLR2 and TLR4 and the intracellular HSP70 were stained and analyzed by flow cytometry, and plasma level of TNF-?, IL-6, IL-10, NO and MDA were measured at following times: before the dexamethasone or placebo were administer(T1), before starting CPB(T2), immediately after aortic declamping(T3), 30min after aortic declamping(T4), 5h after skin closure(T5) and 24h after skin closure(T6). Results Both the HSP70~+ TLR2~+ monocytes and HSP70~+-TLR4~+ monocytes,the plasma concentration of TNF-?, IL-6, NO and IL-10 were upregulated after introduction (P
