Effect of Nanometer Calcium Combined with Tonifying Kidney Herbs the Expression of CaBP-D_(9K) mRNA and Protein in the Intestinal Mucosa of Osteoporosis Rats Due to Kidney Deficiency
- VernacularTitle:纳米钙补肾中药对骨质疏松症大鼠肠黏膜中CaBP-D_(9K)的mRNA和蛋白表达的影响
- Author:
Lu REN
;
Hongxin ZHENG
;
Shuru LIN
- Publication Type:Journal Article
- Keywords:
osteoporosis;
the nanometer calcium combined with tonifying kidney herbs;
calcium bind proteining
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of nanometer calcium combined with tonifying kidney herbs on osteoporosis by studying the expression of CaBP-D9K mRNA and protein in the intestinal mucosa of osteoporosis rats due to kidney deficiency. Method The rat model of osteoporosis due to kidney deficiency was established by ovariectomy. Applying the nanometer calcium and nanometer calcium combined with tonifying kidney herbs (big, middle and small dosage) to the rats for 12 weeks, choosing Gushukang, E2 and Gai’erqiD600 as the control drugs. The expression of the CaBP-D9K mRNA and protein in the intestinal mucosa of the osteoporosis rats due to kidney deficiency was determined by the method of RT-PCR and western blotting. Result There was CaBP-D9K and protein expression in the normal rats intestinal mucosa tissue, which plays an important role in the process of the intestinal calcium absorption. The episode of the rat osteoporosis due to kidney deficiency is related to the change of the CaBP-D9K and protein expression and has influence on the intestinal calcium absorption. Conclusion The nanometer calcium combined with tonifying kidney Chinese herbs can stimulate the calcium absorption of intestine, prevent and treat the osteoporosis due to kidney deficiency through regulating the expression of CaBP-D9K mRNA and protein in the intestinal mucosa.
