EFFECTS OF CILIARY NEUROTROPHIC FACTOR ON CELL CYCLE AND EXPRESSION OF Fos PROTEIN IN ASTROCYTES IN VITRO
- VernacularTitle:睫状神经营养因子对体外培养的星形胶质细胞细胞周期及Fos蛋白表达的影响
- Author:
Xiaojing WANG
;
Zhengli LI
- Publication Type:Journal Article
- Keywords:
Ciliary neurotrophic factor;
Astrocyte;
Cell cycle;
Fos protein;
Cell culture;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ciliary neurotrophic factor on cell cycle and expression of Fos protein in cultured astrocytes from cerebral cortex of rat in vitro Methods Astrocytes from cerebral cortex of rat were cultured in vitro.The cultured astrocytes were treated with ciliary neurotrophic factor(CNTF),and then cell cycle was measured by flow cytometer and Fos protein was examined by means of immunocytochemistry at different intervals. Results At 6h following CNTF treatment,astrocytes cell cycle process was promoted obviously,the percentage of cell in G-1/G-0 cycle phase was reduced,while that of G-2/M and proliferation index(PI) increased.The proliferous effect peaked at 12h,and then gradually declined at 24h,48h,but PI was higher than that of control group.The expression of Fos protein was enhanced from 2h to 24h after CNTF treatment,the pretreatment of dexamethasone inhibited the expression of Fos protein.Conclusion CNTF may promote proliferation and the expression of Fos protein in astrocytes.
