THE DISTRIBUTION AND ORIGIN OF CGRP IMMUNOREACTIVE NERVE TERMINALS IN THE PREPUCE AND FRENULUM OF PENIS
- VernacularTitle:成人和大鼠阴茎包皮及包皮系带内降钙素基因相关肽免疫阳性神经末梢的分布及来源
- Author:
Zhongmin WU
- Publication Type:Journal Article
- Keywords:
The frenulum;
DRG;
CGRP;
FG;
Immunohistochemistry;
Human;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the distribution and origin of calcitonin gene-related peptide(CGRP) immunoreactive nerve terminals in the prepuce and frenulum of penis of human and mice. Methods The CGRP-immunoreactive nerve terminals in the prepuce and frenulum of penis were detected by the method of immunohistochemistry.The fluoro-gold(FG) retrogradely tracing and CGRP immunofluorescence were used to tracing the origin of CGRP-immunoreactive terminals in the penile frenulum of rats. Results In the prepuce and frenulum of adult penis,CGRP-immunoreactive nerve terminals were mainly observed in the basal stratum and spinal stratum of epidermis distributing in a cluster.These nerve terminals were arborescent and beady in appearance.The density of these nerve terminals was significantly higher in frenulum than that in prepuce.The distribution of CGRP-immunoreactive nerve terminals in rats was similar to that of in adult.FG retrogradely tracing method found that the FG retrolabelled neurons were localized in L-6DRG and S-1-DRG.Combined CGRP immunofluorescence method indicated that a number of DRG neurons were showed CGRP-immunoreactive.CGRP-positive neurons were mainly moderate or small-sized,intense immunostained,bright green granules appeared as FITC fluorescence in the cytoplasm.These positive-neurons were arranged in rows or spots among nerve bundles.A half of FG-labelled neurons also showed CGRP-immunoreactive expression in L-6-DRG and S-1-DRG respectively.All of FG/CGRP double-labelled neurons were moderate or small-sized.Conclusion Large numbers of CGRP-immunoreactive nerve terminals were observed in the penile frenulum of human and rats.These terminals were originated from the peripheral branches of CGRP-immunoreactive neurons in L-6-DRG and S-1-DRG of rats.It is suggested that CGRP may take part in the transmission of nociception in the prepuce and frenulum of penis.
