THE CHANGES OF THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR DURING RAT LIVER REGENERATION
- VernacularTitle:血管内皮生长因子在大鼠肝再生过程中的表达变化
- Author:
Wenxue ZHANG
;
Liangzhen ZHAO
;
Yanhong ZHAO
;
Wenling LIU
;
Cunshuan XU
- Publication Type:Journal Article
- Keywords:
Liver regeneration;
Vascular endothelial growth factor(VEGF);
Hepatocytes;
Immunocytochemistry;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Detecting the changes of the expression of vascular endothelial growth factor(VEGF) during rat liver regeneration so as to study the role of VEGF in the course of liver regeneration. Methods Three hundred adult SD rats were randomly divided into three groups:control group,sham operated group and test group.After taking out liver tissue and isolating hepatocytes at different phases after 2/3 rat hepatectomy,the change of VEGF expression was detected by both immunocytochemistry and Western blotting. Results The results of immunocytochemistry showed that VEGF positive cells were little in normal liver.The amounts of immunoreaction positive cells began to increase after 24 h of partial hepatectomy(P
