EFFECTS OF HCY ON THE EXPRESSION OF HCY-2 AND THE PROLIFERATION IN HUMAN FETUS ARTERIAL SMOOTH MUSCLE CELLS
- VernacularTitle:同型半胱氨酸对人主动脉平滑肌细胞HCY-2表达及细胞增殖的影响
- Author:
Danyu LU
;
Guangming WANG
;
Xiao HAN
;
Lina ZHANG
;
Jun WU
;
Guanghui CHEN
;
Ying LI
- Publication Type:Journal Article
- Keywords:
Homocysteine induced gene;
Homocysteine;
Folic acid;
Immunohistochemistry;
Arterial smooth muscle cells;
Fetus
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the homocysteine induced gene(HCY-2) expression in human fetus arterial smooth muscle cells(hASMCs) in culture and explore the effects of homocysteine(HCY) and folic acid on HCY-2 expression and cell proliferation of hASMCs. Methods Immunohistochemistry ABC staining method was used to observe and analyze HCY-2 expression in hASMCs in culture.The image analysis system was used to research of hASMCs quantificationally.The effect of different HCY concentration on the proliferation of hASMCs was investigated by the cell counting. Results Immunoreactive substance of HCY-2 was chiefly found in cytoplasm of hASMCs.The expression of HCY-2 could be affected by HCY concentrations.There was a positive dose-dependent correlation with HCY concentrations in the culture medium.Folic acid increased the expression of HCY-2.The different concentration of HCY enhanced the proliferation of hASMCs,and this enhancement was maximal at the concentration of 1.25 mmol/L of HCY,while the proliferation was decreased when the concentration of HCY was over 1.25 mmol/L.Conclusion HCY increases the expression of HCY-2,and affects the proliferation of hASMCs.HCY is inhibited by folic acid.
