THE EFFECTS OF hTERT AND C-MYC ANTISENSE OLIGODEOXYNUCLEOTIDE ON THE TELOMERASE ACTIVITY IN HL-60 CELLS
- VernacularTitle:端粒酶逆转录酶及c-myc基因反义寡核苷酸对HL-60细胞端粒酶活性的影响
- Author:
Ling SUN
- Publication Type:Journal Article
- Keywords:
Human telomerase reverse transcriptase;
c-myc;
Antisense oligodeoxynucleotide;
Telomerase activity;
TRAP-ELISA,PAGE-silver staning;
HL-60 cells
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human telomerase reverse transcriptase(hTERT) and c-myc antisense oligodeoxynucleotide(ASODN) on the telomerase activity in HL-60 cells,and to explore the relationship between the telomerase activity and the expression of hTERT and c-myc in HL-60 cells. Methods The expression of hTERT and c-myc was sealed by ASODN in HL-60 cells,and was detected by RT-PCR.The telomerase activity was determined with TRAP-ELISA and PAGE-silver staining. Results After treated with ASODN for 72 hours the expression of hTERT and c-myc mRNA was inhibited in different degree,and was the lowest in group of 30??mol/L ASODN.As for the TRAP-ELISA detection,the difference was significant when compared with groups of 10,20,30??mol/L hTERT ASODN and 20、30??mol/L c-myc ASODN with group of untreated(P0.05).PAGE-silver staining detection showed that comparing ASODN groups with SODN groups the telomerase image strips were decreased and was the least in group of 30??mol/L,and the telomerase image strips were also decreased when compared the same density group of hTERT ASODN with c-myc ASODN.Conclusion hTERT and c-myc ASODN may inhibit the expression of the genes respectively and down-regulate the telomerase activity in HL-60 cells.
