Effects of Aralia chinesis L on Proliferation and Function of Fibroblasts
- VernacularTitle:太白楤木对成纤维细胞增殖及功能的影响
- Author:
Danli NIE
;
Lei YI
;
Dajiang CUI
;
Chengzhi YANG
- Publication Type:Journal Article
- Keywords:
Aralia chinesis L;
Fibroblast;
Hyaluronic acid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Aralia chinesis on the proliferation and viability of fibroblasts and on the production of the hyaluronic acid(HA)in the cultured supernatant,and to explore its anti-hepatofibrosis mechanism.Methods NIH3T3 fibroblasts,which were cultured in vitro by routine method and were used as the substitutive model of hepatic stellate cells(HSC),were cultured with the rats serum containing Aralia chinese.The effects of Aralia chinesis serum on the cell proliferation was measured by methabenzthiazuron(MTT)assay and the HA content in cultured supernatant was detected by radioimmunoassay.Results Aralia chinesis serum showed no significant toxicity on NIH3T3 fibroblasts.5 %concentration serum of Aralia chinesis inhibited the cell proliferation and the synthesis of HA significantly(P
