Determination of Hesperidin Content in Weifukang Capsules by HPLC
- VernacularTitle:HPLC法测定胃复康胶囊中橙皮苷的含量
- Author:
Xiaohong LIU
- Publication Type:Journal Article
- Keywords:
Weifukang Capsule;
Hesperidin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of hesperidin in Weifukang capsules by HPLC,and to establish the quality standard of Weifukang.Methods HPLC method was performed on C18 column(4.6 ?200 mm,5 ?m).The mobile phase consisted of acetonitrile-sodium dihydrogen phosphate(0.05 mol/L)-phosphoric acid(200 ∶800 ∶0.5).The flow rate was 1.0 mL/min,column temperature was 25 ℃,and the detection wavelength was 283 nm.Results Hesperidin was linear at the range of 0.38 ?g～1.90 ?g(r=0.999 2)the mean recovery rate was 97.88 %,and RSD was 1.69 %.Conclusion This method is effective and reproducible and provides a basis for the quality control of Weifukang capsules.
