Study of Preparing the Red Cell Reagent Serum by Sepharose 4B Immunoadsorption
- VernacularTitle:Sepharose 4B免疫吸附法制备红细胞试剂血清的研究
- Author:
Jiongcai LAN
- Publication Type:Journal Article
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The absorption of anti-A and anti-B in reagent sera containing non-ABO antibodies,performed by coupling the ABH substance to se- pharose 4B,was introduced.The sera containing both ABO antibodies and anti-D(11),-C(5),-E(5),-c(6), -e(4),-M(1),-N(1),-s(1),-K(1), -Kp~b(1),-Fy~a(1),-Js~b(1)absorbed by this method,were tested then by the ABO-incompatible cells lacking relevant non-ABO antigens,and the panel red cells(O).As a result,all the ABO antibodies were absorbed exclusively,but all the non-ABO antibodies were preserved fullyThe non-ABO antibodies can't give the false positive reaction and their titer can't decrease after repeated absorp- tion.The advantages of this method and the factors that have influeced on effectiveness of the absorption were discussed.
