RP-HPLC Determination of Geniposide in Xiaoyan Tuire Patch
- VernacularTitle:RP-HPLC法测定消炎退热贴中栀子苷的含量
- Author:
Jianwei HAN
;
Ming QIAO
;
Min FU
- Publication Type:Journal Article
- Keywords:
Xiaoyan Tuire Patch;
Geniposide;
RP-HPLC;
determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method to determine the content of geniposide in Xiaoyan Tuire Patch. Method The column of Agilent ZORBAX SB-C18 (4.6 mm?250 mm 5 ?m) was used. The mobile phase was composed of water-acetonitrile-phosphoric acid (86∶14∶0.01). The flow rate was 1 mL/min, with UV detection at 238 nm, and the temperature was 30 ℃. Results The linear range was 0.07～0.70 ?g (r =0.99997). The average recovery was 98.60% and the RSD was 1.96%. Conclusion The method is simple, sensitive and accurate, and can be used for the determination of Xiaoyan Tuire Patch.
