Effect of Buyang Huanwu Decoction and its active fractions on caspase expression after cerebral ischemia-reperfusion in rats
- VernacularTitle:补阳还五汤及其有效部位对大鼠脑缺血再灌注后caspase表达的影响
- Author:
Shuping ZHANG
;
Yan LIANG
;
Changqing DENG
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction(BHD);
cerebral ischemia-reperfusion;
cysteine-aspirate protease(caspase);
reverse transcription-polymerase chain reaction(RT-PCR)
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Buyang Huanwu Decoction(BHD) and two kinds of fractions extracted from BHD on expression of caspase-1,caspase-3,and caspase-8 mRNA after cerebral ischemia-reperfusion in rats.Methods The model of cerebral ischemia-reperfusion was induced by the middle cerebral artery occlusion(MCAO) in rats,via string ligation of arteria carotis interna.The expression of caspase-1,caspase-3,and caspase-8 mRNA in cerebral ischemic tissues was assessed by reverse transcription-polymerase chain reaction(RT-PCR).Results The expression of caspase-1,caspase-3,and caspase8 mRNA in cerebral tissues was significantly increased after 22 h reperfusion following 2 h cerebral ischemia in model group(P
