Effect of pronuciferine on apoptosis of human umbilical vein endothelium cells induced by angiotensin Ⅱ
- VernacularTitle:前荷叶碱对血管紧张素Ⅱ诱导人脐静脉内皮细胞凋亡的影响
- Author:
Aihua CHEN
;
Hua XIAO
;
Zhiliang LI
;
Jinjia WU
;
Aimin JI
- Publication Type:Journal Article
- Keywords:
pronuciferine;
human umbilical vein endothelium cells(HUVECs);
angiotensinⅡ(AngⅡ);
cell apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pronuciferine on apoptosis of cultured human umbilical vein endothelium cells(HUVECs) induced by angiotensin Ⅱ(AngⅡ).Methods HUVECs cell line ECV304 was cultured in vitro,pretreated with Captopril(10 ?mol/L) or pronuciferine 10,1,0.1,0.01 ?mol/L for 30 min,respectively,then treated with AngⅡ(1 ?mol/L).Cell-morphosis was observed by light microscope.Cells viability was assessed by MTT assay.Production of nitric oxide(NO),activities of total nitric oxide synthase(tNOS),and inducible nitric oxide synthase(iNOS) were measured by colorimetry.Apoptosis rate was measured by Flow Cytometer(FCM).Results AngⅡ induced typical endothelial cell apoptosis and the apoptosis rates were significantly higher than those of the control group((P
