Genetic transformation of Erigeron breviscapus induced by particle gun with a few affecting factors
- VernacularTitle:基因枪介导灯盏花遗传转化及几个影响因素的研究
- Author:
Lu QIU
- Publication Type:Journal Article
- Keywords:
Erigeron breviscapus(Vant.) Hand.-Mazz.;
particle gun;
genetic transformation;
bombarded materials;
bombarding pressure;
Gentamicin(Gent.)
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To get genetic transformated Erigeron breviscapus plantlet with the antiinsect and antifungi effects.Methods The plasmid with gene RBBI2-3 was produced by PCR,middle carrier PBS and plant carrier YY46.By using the particle gun to bombard E. breviscapus,genetic transformation was realized.It was done with the control experiment on bombarded materials,bombarding pressures,and Gent.sensitivity.Results The result of genetic transformation by using true leaves as bombarded material was the best;Ⅱ-type callus were the next in order;the roots,stems,and Ⅰ-type callus were the last in the result.The bombarding pressure of 8.96?10~6 Pa was the best to induce true leaves forming adventitious bud.The result of using the pressure of 7.58?10~6 Pa and 8.96?10~6 Pa to bombard Ⅱ-type callus did not show any difference and was next to true leaves.The best result is using 50 ?g/mL Gent to choose the E.breviscapus of genetic transformation.Conclusion The paddy RBBI2-3 transformed into the genetic transformation on E.breviscapus by particle gun bombarding is the best.The genetic transformated E.breviscapus plantlet has been obtained.
