Cloning and sequencing of human apM1 gene
- VernacularTitle:人脂联素基因的克隆及序列分析
- Author:
Bingrong LI
- Publication Type:Journal Article
- Keywords:
apM1 gene;
Cloning,Molecular;
Reverse transcriptase polymerase chain reaction;
Sequence analysis,DNA
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone human apM1 gene for the study molecular mechanism of the function of adiponectin.Methods: RT-PCR method was used to isolate,from the total RNA of human adipose tissue,cDNA fragment encoding adiponectin,which was linked into pGEM-T Vector,then amplified,and cloned.The sequence was analyzed to identify the recombinant plasmids.Results: The result of sequencing indicated that the sequence of the cloned cDNA was identical to that of the corresponding parts of the mRNA for human apM1 in GeneBank except 2 nucleotides.Conclusion: Human apM1 cDNA gene was successfully cloned.
