Expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in BCG
- VernacularTitle:多房棘球绦虫Em14-3-3抗原编码基因在BCG中的表达
- Author:
Hong WANG
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
Recombinant Em14-3-3 protein.;
BCG;
Gene expression
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in Bacillus Calmette-Guerin(BCG).Methods: The recombinant plasmid pBCG-Em14-3-3 was introduced into BCG by electroporation and the bacteria with pBCG-Em14-3-3 were cultured to logarithm growth period,then induced daily by heating at 45℃ for 30min in the last three days.The expression products were analyzed by SDS-PAGE and Western blot.Results: The Em14-3-3 recombinant protein was successfully expressed by pBCG-Em14-3-3 in BCG as an obvious band of 27kDa by SDS-PAGE,which accounted for 11% of total bacterial protein in BCG.Conclusion: The pBCG-Em14-3-3 could be expressed efficiently in BCG and be combined with specific antibody in mice sera,which suggested that the Em14-3-3 recombinant protein expressed by rBCG-Em14-3-3 vaccine was of specific antigenicity.
