Expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro
- VernacularTitle:HSP90在乳腺癌细胞株ZR-75-30中的表达及对粘附侵袭能力的影响
- Author:
Hongxia LUO
- Publication Type:Journal Article
- Keywords:
Breast cancer;
HSP90;
Geldanamycin;
Metastasis
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro and the mechanism of anti-invasion.Methods:The protein expression level of HSP90 in ZR-75-30 before and after treatment with HSP90 inhibitor Geldanamycin was detected by Western-blotting;The effects of GA on the proliferation,invasion and motility of human breast cancer cell line ZR-75-30 in vitro were explored respectively by MTT colorimetric assay and transwell chamber.Results:After treatment with GA the protein expression level of HSP90 in ZR-75-30 was decreased,and the proliferation of ZR-75-30 cells was obviously inhibited in a dose and time-dependent manner.Compared with the control guoup,the adhesion,invasion and motility abilities of the ZR-75-30 cells were decreased significantly(P
