Generation and characterization of fibre modified adenovirus vectors
- VernacularTitle:新型腺病毒载体的制备及其特性研究
- Author:
Feng QIU
- Publication Type:Journal Article
- Keywords:
Adenovirus vector;
RGD peptid;
Integrin
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In the present study,We developed and assess an integrin-targeted fiber-mutant Ad vector containing the Arg-Gly-Asp(RGD) peptide motif on the fiber knob for the transfection of melanoma cell lines.Methods: We constructed RGD-Adv by in vitro ligation method,and investigated the efficiency of gene transdution in melanoma cells,studied its stability,in vitro,and in vivo toxicology.Results: For in vivo and in vitro,gene transfer the RGD-Adv more efficient than the vector containing wild type fiber via an CAR independent cell entry pathway.Conclusion: These results revealed that gene delivery by RGD-Adv was more efficient than that by conventional Adv and suggest that av-integrin-targeted Adv will be of great utility for gene therapy and gene transfer experiments.
