Repair of articular cartilage defects by transplantation of PLGA compounded with mesenchymal stem cells modified by hIGF-1 gene
- VernacularTitle:hIGF-1基因修饰骨髓基质干细胞复合PLGA移植修复关节软骨缺损
- Author:
Xingpo DING
- Publication Type:Journal Article
- Keywords:
Human insulin-like growth factor;
Mesenchymal stem cells-1;
Articular cartilage defects
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To invest the repair effect of articular cartilage defects by transplantation of PLGA compounded with mesenchymal stem cells modified by hIGF-1 gene.Methods: After the amplification by polymerase chain reaction(PCR) from pcDNA3.1-hIGF-1,the human insulin-like growth factor-1(hIGF-1) fragment was inserted to plasmid pIRES2-EGFP to get recombinated vector pIRES2-EGFP-hIGF-1,which followed by transfection of restructured vectors to mesenchymal stem cells and determination the expression of hIGF-1 gene.The gene-modified MSCs were compounded with PLGA and then cultured in standard condition.Then,the transplantation of graft to the defect positon of articular cartilage was performed.After the transplantation,the specimens was obtained in different time and the repair was inspected.Results: The reconstructed vector contained hIGF-1 and GFP genes was constructed successfully and high efficiency expression of hIGF-1 was detected in target cells.Compared to the control groups,the group of transplantation of graft compounded with MSCs modified hIGF-1 greatly improved the effect of articular cartilage defects.Conclusions: IGF-1 gene is one of the ideal candidate genes for the gene modification of MSCs,the seed cells of cartilage tissue engineering and the gene-modified MSCs can improved effective the repair of articular cartilage defects.
