Research on the experiment of eliciting specific antitumor immunity by fusion of dendritic cell with gastric cancer cells in vitro
- VernacularTitle:树突状细胞与胃癌细胞融合诱导抗肿瘤免疫反应的体外实验研究
- Author:
Minman NING
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Gastric cancer;
Cell fusion
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: By hybrid technology,to study the biological characteristics and antitumor function of gastric cancer-dendritic fusion cells through the fusion of dendritic cells from cord blood with gastric cancer cells SGC7901,Method: DCs from cord blood were fused with SGC7901 in the presence of 50% polyethylene glycol.The morphology and phenotype of DCs,SGC7901 and DCs/SGC7901 were respectively observed by SP immunocytechemical staining.The ability of DCs/SGC7901 which stimulated proliferation of autologous T lymphocytes was assessed by MTT,and the kill rate of cytotoxic T lymphocytes(CTL) to SGC7901 induced by FCs(fusion cells) was evaluated through cytotoxic test.Result: DCs were successfully fused with SGC7901 in the presence of PEG,FCs expressed CEA and HLA-DR two phenotypes.Moreover,fusion cells had greater ability to stimulate proliferation of T lymphocytes than DCs?DCs+SGC7901,SGC7901,and the kill rate of FCs(21.4%?0.0165%) was greatty high than that of DCs(16.3%?0.0015%),DCs+SGC7901(15.3%?0.0044%),SGC7901(11.2%?0.0156%).Conclusion: DCs from cord blood can be successfully fused with SGC7901 in the presence of PEG,and FCs can effectively stimulate T lymphocytes to elicit antitumor immunity effect.
