Sero-pharmacological Studies on Guyanding in Promoting the Proliferation of Vascular Endothelial Cells
- VernacularTitle:骨炎定促进血管内皮细胞增殖的血清药理学研究
- Author:
Yanming CAO
;
Haibo XU
;
Wei ZHANG
;
Jizhang CHEN
- Publication Type:Journal Article
- Keywords:
OSTEOARTHRITIS/TCD therapy;
GUYANDING DECOCTION/drug effects;
CELL CULTURE;
RABBITS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To observe the effect of Guyanding(GYD)decoction,a prescription with the actions of tonifying kidney,strengthening Qi and activating blood on proliferation of vascular endothelial cells(VEC).【Methods】New Zealand rabbits were randomized into blank control groups and GYD decoction groups in the dosages of 2.5,5 and 10g/kg respectively.GYD in the dosage of 10g/kg was given to rats for 7 successive days,and blood sampling was collected 0.5,1,1.5,2,2.5,3,3.5 and 4 hours after the last administration to prepare serum containing GYD.Then human umbilical vein endothelial cells were cultured with serum containing GYD,and the effects of GYD-containing serum at different time points on the proliferation of VEC were observed by methylthiazolyltetrazolium(MTT)assay.GYDcontaining serum collected 2h after the last administration was partially inactivated and its effect on VEC proliferation was compared with that of non-inactivated GYD-containing serum.Meanwhile,the effect of non-inactivated GYD-containing serum on VEC proliferation was observed 12,24 and 36 hours after the culturing.GYD in three different dosages was given to rats for 7 successive days,and serum containing GYD collected 2h after the last administration was prepared to observe the effect of GYD in different doses on VEC proliferation.【Results】Serum containing high-dose GYD collected 0.5～4h after the administration had obvious effect in promoting VEC proliferation(P
