Determination of cinobufagin and resibufogenin in Xinkening Capsules by HPLC
- VernacularTitle:HPLC测定心可宁胶囊中华蟾酥毒基和脂蟾毒配基的含量
- Author:
Shuying LI
;
Hailin DONG
;
Xiao LIANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Xinkening Capsules;
cinobufagin;
resibufogenin
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method of determining cinobufagin and resibufogenin in Xinkening Capsules(Venenum Bufonis,etc.). METHODS: HPLC was applied to determining cinobufagin and resibufogenin.The determination was carried out using an ODS column with a solvent system: methanol-water(50∶40).The flow rate was 1.0 mL/min,detection wavelength was at 296 nm. RESULTS: The linear ranges of cinobufagin and resibufogenin were 3.5 ?g-0.28 ?g and 3.75 ?g-0.30 ?g,respectively.The average recoveries of cinobufagin and resibufogenin were 99.86%(RSD=1.8%,n=5),and 99.84%(RSD=1.2%,n=5),respectively. CONCLUSION: This method is simple,accurate with strong specificity and can be used to control the quality of Xinkening Capsules.
