Effect of bifidobacterium on the differentiation and development of DC
- VernacularTitle:树突状细胞分化发育过程中双歧杆菌的作用研究
- Author:
Fei XIONG
- Publication Type:Journal Article
- Keywords:
Bifidobacterium;
Dendritic Cells
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of bifidobacterium on the production and morphology of dendritic cells(DC) in vitro and in vivo.Methods: In vivo,the mice were administrated orally with live bifidobacteria,heat-killed bifidobacteria(1?109CFU/ml),with spent culture supernatant(SCS) or saline normal respectively,and their intestinal samples were used to evaluate the production of intestinal DC by immunohistochemical method.In vitro,the monocyte was induced by heat-killed bifidobacterium alone,to find out whether it could change cell morphology and become DC,Phase-contrast microscope morphological observation was made.On the other hand,normal adult immature DCs were induced by monocyte cultured in granuloeyte macrophage-colony stimulating factor(GM-CSF) and interleukin-4(IL-4),and they were exposed to various dosages of heat-killed bifidobacterium(100ug/ml,10ug/ml) in experimental groups on the 7th day,while the control group was exposed without heat-killed bifidobacterrium,LPS at 1ug/ml was added to some cultures as positive control.The morphology of DC was observed by phase-contrast microscope and electron microscope.Results: Under the microscope,DCs were present in the intestinal lamina propria of mice,the sizes of which were varied and the appearances were irregular.Counting showed that the bifidobacterium could increase the number of DCs in the small intestine of mice.There was significant difference between the control group and the live bifidobacterium group,the heat-killed bifidobacterium group and SCS group(P
