Study of EPO pretreatment's effect on expression of TNF-? gene in cultured cardiac myocytes with hypoxia/reoxygenation injury and the possible mechanism
- VernacularTitle:EPO预处理对培养心肌细胞缺氧/复氧损伤TNF-?表达的影响及机制
- Author:
Chuan QIN
;
Yingbin XIAO
;
Qianjin ZHONG
- Publication Type:Journal Article
- Keywords:
Myocardium Eryghropoietin Tumor necrosis factor NF-kappa B Myocardial reperfusion injury Animal testing alternative
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study is to investigate the effect of EPO pretreatment on TNF-? expression in cultured cardiac myocytes with H/R and to explore the possible NF-?B signal transduction mechanism. Methods The model of cultured cardiac myocytes with H/R was established and the cardiac myocytes were divided into 4 groups, including EPO group (treat with EPO 10?U/ml 24?h before H/R), EPO+PDTC group (treat with EPO 10?U/mL and PDTC 5??g/ml 24?h before H/R), PDTC group (treat with PDTC 5??g /ml 24h before H/R) and control group. Change of TNF-? gene expression before and after H/R in cardiac myocytes was detected with RT-PCR and western blot. Change of NF-?B activity before and after H/R in cardiac myocytes was assayed with EMSA. Results Before H/R, there was no significant difference in TNF-? mRNA and protein expression between the 4 groups and after H/R, TNF-? mRNA and protein expression increased significantly in the 4 groups compared to control group before H/R. After H/R, TNF-? mRNA and protein expression was lower in EPO group than in the other 3 groups. Before H/R, NF-?B activity was higher in EPO group than in the other groups. After H/R, NF-?B activity increased significantly in all the 4 groups compared to the control before H/R and NF-?B activity was lower in EPO group than in the other groups. Conclusion EPO pretreatment inhibited the upregulation of TNF-? gene expression after H/R in cardiac myocytes, which might be related to the inhibition of NF-?B activation; EPO pretreatment might inhibit the activation of NF-?B after H/R through the negative feed-back mechanism of NF-?B activation.
