Determination of Baicalein in Tangtai Capsule by HPLC
- VernacularTitle:HPLC法测定糖泰胶囊中黄芩苷的含量
- Author:
Qiuhong ZHANG
;
Bin YAN
;
Chao LIN
- Publication Type:Journal Article
- Keywords:
HPLC;
Tangtai Capsule;
baicalein;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A HPLC method to determine the content of Baicalein in Tangtai capsule was established. Method The separation was performed by Zorbax C18 column with acetonitrile and 0.5% phosphorice acid. The elution program was 1~15 min, 15%～60% acetonitrile. The flow rate was 1 mL/min and column temperature was 25 ℃. Content was detected at a wavelength of 280 nm. Result The linear range was 0.936～18.72 ?g, regression:Y =4849.53X-0.9593 (r=0.9999). The average recovery rate was 98.29% and RSD was 0.97% (n =5). Conclusion The method is simple, accurate and reproductive, and can be used for determining Baicalein in Tangtai capsule.
