Reversal effect on multidrug resistance and effect on intracellular on Ca~(2+) concentration of HL60/HT cells by psoralen
- VernacularTitle:补骨脂素对HL60/HT耐药细胞的逆转作用及对细胞内钙离子浓度的影响
- Author:
Tiange CAI
;
Yu CAI
;
Bing CHEN
- Publication Type:Journal Article
- Keywords:
psoralen;
multidrug resistance;
reverse;
HL60/HT cells
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the reversal the effect on multidrug resistance (MDR) and the effect on Ca~(2+) concentration of HL60/HT cell by psoralen, and observe its possible mechanism. Methods The cytotoxic effect of chemotherapeutic agents was determined by MTT assay, intracellular harringtonine (HT) content was assayed by HPLC, and intracellular Ca 2+ concentration in different periods and at different time points was detected by laser confocal microscope. Results Psoralen could reduce IC_ 50 value of HT to HL60/HT cells and increase HT content in cells in different degrees at the doses of 1-20 ?mol/L. Psoralen could influence Ca 2+ concentration with negative correlation at different time points (24, 48, and 96 h) at the doses of 1-20 ?mol/L, and influence Ca 2+ concentration with negative correlation at different times (5, 10, 15, 20, and 25 min) in the doses of 10 and 20 ?mol/L. Conclusion Psoralen can reverse MDR of HL60/HT cells, its reversal mechanism is associated with influence of intracellular Ca~(2+) concentration and the increase of intracellular accumulation of HT.
