Effects of dexamethasone on expression of glucocorticoid receptor in human monocyte cell line THP-1
- VernacularTitle:地塞米松对人单核细胞糖皮质激素受体蛋白表达的影响
- Author:
Bo LI
;
Xiangjun BAI
;
Haiping WANG
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Glucocorticoid;
Receptor;
Glucocorticoid resistance
- From:
Chinese Journal of Emergency Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of dexamethasone on the expression of glucocorticoid receptor (GR ?, GR ?) in human monocyte cell line THP-1.Methods Cultured THP-1 cells were stimulated by dexamethasone with different concentrations and for different durations. The GR ? and GR ? protein expressions were examined by Western blotting. Results The expressions of GR ? and GR ? were observed in stimulated and unstimulated THP-1. The quantity of GR ? expression was reduced by dexamethasone treatment in a time-dependent manner. The quantity of GR ? expression was increased by dexamethasone treatment in time- and dose-dependent manner. Conclusion Dexamethas one stimulation time-dependent reduce GR ? expression in THP-1 cell. Dexamethasone stimulation time- dependent and dose-dependent increase GR ? expression in THP-I. The expressions of GR a and GR ? were regulated by glucocorticoid.
