NEUROTOXICITY OF ROTENONE ON DOPAMINERGIC NEURONS IN CULTURED SLICES OF THE MIDBRAIN IN THE RAT
- VernacularTitle:鱼藤酮对体外培养的大鼠中脑脑片多巴胺能神经元的毒性作用
- Author:
Yan SHANG
;
Liang LI
- Publication Type:Journal Article
- Keywords:
Rotenone;
Dopamine;
Parkinson disease;
Dopaminergic neurons;
Tissue culture;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the early neurotoxicity of rotenone on dopaminergic neurons and explore an ideal tissue model. Methods A long-term midbrain slice culture system of SD pup was established according to the interface tissue culture method.After rotenone was added for some time,its toxic effects on the whole slices and the dopaminergic neurons were identified through the measurements of lactate dehydrogenase(LDH) released into the medium from the slices and dopamine(DA) content from the cultured tissue,as well as the observations of immunohistochemistry for tyrosine hydroxylase(TH). Results In those cultures exposed to rotenone for 24 h,the level of DA in tissue dramatically decreased with the concentrations rising.The processes of TH-positive neurons in slices demonstrated some morphological changes,such as appearance of string of beads,reduce of numbers and even disappearance.The content of dopamine in tissue was dominantly decreased with 5 nmol/L rotenone for 14 days,although its cellular morphology was not seen to change.Conclusion Long-time stable midbrain slice culture system has been set up successfully.The neurotoxicity of rotenone on the whole slices and dopaminergic neurons shows a dose-dependent manner.The functional damages on the neurons may be earlier than their morphological changes,of which the injury in the processes of neurons seems to be an early characteristic.
