THE EXPRESSION OF GLUCOSE REGULATING PROTEIN 78 IN THE HEPATOCIRRHOSIS INDUCED BY CARBON TETRACHLORIDE
- VernacularTitle:四氯化碳诱导性肝硬化大鼠肝组织葡萄糖调节蛋白78表达的研究
- Author:
Xiaohong WANG
;
Weiguang ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatocirrhosis;
Glucose regulating protein 78(GRP78);
Endoplasmic reticulum stress;
Carbon tetrachloride;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the expression of glucose regulating protein 78(GRP78) in livers of carbon tetrachloride(CCl-4) induced hepatocirrhosis rats.Methods Healthy adult male Wistar rats were randomly divided into control group(n=5)and hepatocirrhosis group(n=5).Oily solution with 0.12ml per 100g rat weight was subcutaneous injected in control group,and 60% carbon tetrachloride oily solution with 0.3ml per 100g rat weight were subcutaneous injected in hepatocirrhosis group for 20 weeks.At the first day of 21 weeks,all rats were killed.Each blood was collected from right ventricle and some hepatic function was measured.The morphological structure in hepatic middle lobule were observed through the staining of H E and Sirius red.The expressions of GRP78 was detected by immnunohistochemistry,Western blotting and RT-PCR.Results The serum ALT in 20 weeks hepatocirrhosis group(278.2?88.42) was evidently higher than that in control group(154.8?9.94,t=3.10,P
