EFFECT OF PINEAL GLAND AND MELATONIN ON THE DEAD SIGNAL PATH OF THYMOCYTE IN RAT
- VernacularTitle:松果体及其褪黑素对大鼠胸腺细胞死亡信号通路的影响
- Author:
Ruixiang ZHOU
- Publication Type:Journal Article
- Keywords:
Pinealectomy;
Melatonin;
Fas/FasL;
Caspase;
Apoptosis;
Thymus;
RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pinealectomy and melatonin on the dead signal path of thymocyte in rat. Methods SD rats were divided into 5 groups:normal control,sham-operated control,pinealectomy,pinealectomy+melatonin injection 7.5 mg/(kg\5d);and pinealectomy+melatonin injection 15 mg/(kg\5d).The animals were sacrificed at 4 or 8 weeks after operation.Fas/FasL\,caspase-8\,caspase-12 positive cells in thymus were stained with immunocytochemical ABC method,measured by compute image analysis.The TNF-? expression in thymocyte which was influenced by melatonin was tested by RT-PCR.(Results The) area of Fas positive cells in cortex was increased significantly at 4 weeks after Px,furthermore,this trend continued to enlarge 8 weeks after Px.The result of FasL staining was similar to the Fas.The area of caspase-8 positive thymic T cells increased significantly in both cortex and medulla 8 weeks after Px,but the difference showed no significance on caspase-12.Melatonin administration attenuated the effect caused by Px.The TNF-? expression in cultured rat thymocyte was decrease by melatonin administration.Conclusion Pineal gland can regulate the apoptosis of rat thymocyte by inflence the dead signal path.Rejuvenation of degenerative thymus could be observed by melatonin administration.
