THE GROWTH INHIBITION AND THE APOPTOSIS OF HeLa CELLS INDUCED WITH TCS
- VernacularTitle:天花粉蛋白抑制HeLa细胞生长及诱导细胞凋亡之机制探讨
- Author:
Jinfeng HE
;
Jicheng LL
- Publication Type:Journal Article
- Keywords:
Trichosanthin;
Apoptosis;
Cell cycle;
Caspases;
Western blotting;
HeLa cells
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of the current study is to investigate the growth inhibition and the apoptosis of HeLa cells induced with TCS,and to explore the underlying molecular mechanism.Methods The antiproliferative effect of TCS on HeLa cells was measured with CCK-8.The FCM analysis was carried out to examine the effect of TCS on the cycle distribution of HeLa cells.Electron microscopy and FCM analysis were employed to observe the apoptosis of HeLa cells.The protein expression of caspase-3 was determined by Western blotting.The activity of caspase-3,8,9 was determined by a caspases colorimetric assay kit.Results 1.The proliferation of HeLa cells was significantly inhibited by TCS;2.TCS led a S-phase arrest in cell cycle;3.HeLa cell apoptosis was induced with TCS,which was characterized by chromatin condensation and apoptotic bodies.And the apoptotic rate of HeLa cells increased with time;4.The expression of caspase-3 protein was decreased significantly(P
