THE RESEARCH ON THE PROLIFERATION AND DIFFERENTIATION OF MOUSE PRIMORDIAL GERM CELLS IN VITRO
- VernacularTitle:小鼠原始生殖细胞体外增殖与分化的研究
- Author:
Yan SUN
;
Xiaolin SHI
- Publication Type:Journal Article
- Keywords:
Primordial germ cell(PGC);
Proliferation;
Embryoid body(EB);
Differentiation;
Cell culture;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research on the proliferation and differentiation in vitro of primordial germ cells. Methods We got plenty of primordial germ cells(PGCs) by co-culture of PGCs with sertoli cells(SCs).The primary cells formed embryoid bodies(EBs) by suspension culture.furthermore,these EBs were plated on gelatin-coated culture plates for spontaneous differentiation. Results We got plenty of PGCs by coculture.Primary PGCs and passaged PGCs for histochemistry staining with AKP and immunohistochemistry staining for SSEA-1 display positive character.The EBs by suspension culture differentiated into epithelial-like,fibroblast-like and neuronal-like cells in vitro. Conclusion Primordial germ cells can proliferate and keep the character of stem cells by co-culture with SCs.PGCs can form EBs by suspension culture,most of these EBs can differentiate into neuronal-like cells,they also can differentiate into epithelial-like and fibroblast-like cells in vitro.
