Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines
- VernacularTitle:RNA干扰技术抑制切除修复交叉互补基因1对卵巢上皮性癌细胞顺铂敏感性的影响
- Author:
Guoyan LIU
;
Quanxin QU
;
Ruoran MI
;
Jing QI
- Publication Type:Journal Article
- Keywords:
RNA interference;
ERCC1 gene;
Ovarian neoplasms;
Cisplatin
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study changes of cisplatin sensitivity by RNA interfering the excision repair cross-complementing (ERCC) 1 gene in ovarian cancer cell lines. Methods The small interference RNA (siRNA) targeting ERCC1 gene was designed and synthesized by transcription in vitro, and transfected to ovarian cancer cell line ES-2. The mRNA and protein of ERCC1 were evaluated by means of RT-PCR, western blot and immunocytochemistry. The changes of cisplatin sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTT) assay. Results In ES-2 cell, the mRNA and protein levels of ERCC1 were dramatically decreased 24, 48 and 72 hours after transfection. The sensitivity to cisplatin of ES-2 cell line was increased by 53.88 times after disturbing the ERCC1 gene. Conclusion The sensitivity to cisplatin of ovarian cancer cell lines ES-2 could be enhanced by RNA interfering ERCC1 gene.
