Determination of the Content and Dissolution of Hymecromone in Compound Dantong Capsules by HPLC
- VernacularTitle:复方胆通胶囊中羟甲香豆素的含量及溶出度的测定
- Author:
Li ZHANG
;
Jing ZHU
- Publication Type:Journal Article
- Keywords:
Compound Dantong Capsule;
Hymecromone;
HPLC;
Content determination;
Dissolution
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of hymecromone content and dissolution in Compound Dantong Capsules by HPLC.Methods The content and dissolution of hymecromone was determined by HPLC.The chromatographic conditions were: CAPCELL PAK C18 Column(5 ? m,4.6 mm? 150 mm),methanol-0.01 mol/L phosphate buffer solution(465 ∶ 535) as mobile phase,detection wavelength at 365 nm and the injected volume being 10? L.Dissolution test was performed with 150 mL of water as dissolving medium and with a rotation speed of 100 r? min-1.Results A good linearity of hymecromone was in the range of 0.25 ? g/mL～ 1.75 ? g/mL(r=0.9989),and the average recovery was 99.4 %,RSD=1.60 %(n=5).The dissolutions of 3 batches of samples in 45 minutes were higher than 70 % of the labeled amount,which accorded with the standard.Conclusion This method was simple and accurate,has no interference of other components,and can be used for the quality control and evaluation of Compound Dantong Capsules.
