Determination of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinnaozhi Capsule by HPLC
- VernacularTitle:HPLC同时测定心脑治胶囊中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1含量
- Author:
Qiuhong ZHANG
;
Bin YAN
;
Tao JIANG
- Publication Type:Journal Article
- Keywords:
Xinnaozhi Capsule;
notoginsenoside R_1;
ginsenoside Rg_1 and Rb_1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method of determining notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 contents in Xinnaozhi Capsule(Radix et Rhizoma Notogineseng,Hirudo,Eupolyphaga seu steleophage). METHODS: HPLC was used to determine notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinnaozhi Capsule.The separation was performed by Zorbax C_(18) colunm with acetonitrile and water using a gradient program at UV 203nm at room temperature.The elution program was 0-3 min,20%-25% acetonitrile,3-15 min,25%-45% acetonitrile. RESULTS: 3 saponins were separated well.Average recoveries were 102.93% for notoginsenoside R_1(RSD=(1.26%));105.68% for ginsenoside Rg_1(RSD=1.52%);104.34% for ginsenoside Rb_1(RSD=0.70%),respectively. CONCLUSION: The method is simple and rapid and with satisfactory results.It is suitable for quality control of Xinnaozhi Capsules.
