Simultaneous determination of puerarin and tanshinone Ⅱ A in Xinkeshu Capsule by HPLC
- VernacularTitle:HPLC同时测定心可舒胶囊中葛根素、丹参酮ⅡA含量
- Author:
Bing LIU
;
Yongbo XU
;
Ronghua WANG
- Publication Type:Journal Article
- Keywords:
puerarin;
tanshinone ⅡA;
Xinkeshu Capsules;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine puerarin and tanshinone ⅡA in Xinkeshu Capsule(Radix Puerariae Lobatae,Radix et Rhizoma Salviae Miltiorrhizae) by HPLC simultaneously. METHODS: HPLC C_(18) column was used with methanol-water(85∶15) as the mobile phase. The flow rate was 1.0 mL/min,and the wave length was at 250 nm. RESULTS: There were good liner relationships for puerarin and tanshinone ⅡA with the sample input quantity at the range of 21-63 ?g(r=0.999 8) and 20.02-60.06 ?g(r=0.999 7),respectively.The average recoveries of puerarin and tanshinone ⅡA were 97.7% and 97.0%,respectively. CONCLUSION: This method is simple,accurate,effective and suitable for the quality control of Xinkeshu Capsules.
