Study on Serum Pharmacochemistry of Shuangshenningxin Fang and its Protective Effects on Cultured Cardiomyocytes Injured by Hypoixa and Reoxygenation
- VernacularTitle:双参宁心方血清药物化学和抗心肌细胞缺氧/复氧损伤的实验研究
- Author:
Yuyang SUN
;
Jianxun LIU
;
Xiaoyang WU
- Publication Type:Journal Article
- Keywords:
Shuangshenningxin Fang;
serum pharmacochemistry;
cardiomyocytes;
hypoixa and reoxygenation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
s:Objective To study the effective ingredients of Shuangshenningxin Fang (SSNX). Methods Based on analyzing the HPLC fingerprints of rat samples obtained after orally taking SSNX, the ingredients absorbed into blood were identified. The cultured cells were subject to hypoxia and reoxygenation. The leakage of lactic dehydrogenase (LDH) from cardiomyocytes was measured. Results Fifteen ingredients were detected after oral administration of SSNX. Dl-tetarhydropalmatine (Dl-THP), salvianolic acid B (Sal B), ginsenosides (Rg1) and dehydeocorydaline (DHC) were identified. Rg1 and DHC reduced the LDH leakage (P
