HPLC Determination of Cinobufagin and Resibufogenin in Jiuxin Tablet
- VernacularTitle:RP-HPLC法测定救心丸中华蟾酥毒基和脂蟾毒配基的含量
- Author:
Taishan CAO
;
Jiepeng LUO
- Publication Type:Journal Article
- Keywords:
Jiuxin Tablet;
Cinobufagin;
Resibufogenin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estalish an accurate method for the determination of cinobufagin and resibufogenin in Jiuxin Tablet. Method Waters Symmetry Shield RP18 (3.9 mm?250 mm, 5 ?m) column in an oven at 30 ℃ was need, with a mobile phase consisting of acetonitrile-0.5mol/L kalium dihydrogen phospate (phosphoric acid preparating pH=3.2) (42∶58) and a UV detector at 296 nm, the flow rate was 1.0 mL/min. Results The linear ranges of Cinobufagin was 0.0696～0.8352 ?g. The RSD of measurement precision test was 0.53%, the average recovery was 101.86% (RSD=1.81%, n=5), the linear ranges of Resibufogenin was 0.1576～0.9456 ?g, the RSD of measurement precision test was 0.15%, the average recovery was 102.07% (RSD=1.62%, n =5). Conclusion The method was simple and accurate, and can be used for the quality control of Jiuxin tablet.
