Effect of serum containing total flavonoids from roots of Daphne genkwa on cell immunity in mice
- VernacularTitle:芫花根总黄酮含药血清对小鼠细胞免疫功能的影响
- Author:
Xiaowen GAO
;
Weifa ZHENG
;
Yecheng PENG
- Publication Type:Journal Article
- Keywords:
total flavonoids from the roots of Daphne genkwa Sieb.et Zucc.(TFRD);
serum pharmacology;
cell immunity
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for assaying total flavonoids from the roots of Daphne genkwa(TFRD) in serum of mice and to elucidate the effect of mice serum containing TFRD on cell immunity in mice.Methods TFRD Concentration in serum was determined from the mice received single ig TFRD at certain time intervals using HPLC method.The effects of TFRD serum on lymphocyte proliferation,killing activities of NK and LAK cell,and phagocytic activity of macrophage were detected by MTT method.Results TFRD in serum reached its highest concentration in 20—30 min after ig admi-(nistration.) TFRD-containing serum significantly improved the proliferation of lymphocyte,enhanced the killing activities of NK and LAK cells,and enforced the phagocytic activity of macrophage.Conclusion(TFRD-)containing serum is an effective agents for enhancing cell immunity in mice.
