Antagonizing effect of paeonol on peroxynitrite-induced rat osteoblast apoptosis
- VernacularTitle:丹皮酚拮抗过氧亚硝基阴离子致大鼠成骨细胞凋亡的作用
- Author:
Jinsheng QI
;
Hui WANG
;
Tao NIU
;
Bingshan ZHANG
;
Xiaolin ZHANG
- Publication Type:Journal Article
- Keywords:
paeonol;
peroxynitrite;
osteoblast;
apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of paeonol antagonizing peroxynitrite(ONOO~-) on osteoblast apoptosis of rat in vitro.Methods Osteoblasts of newly born rat's skull were separated and cultured by tissue mass method.ONOO~-was produced by chemical synthesis with a quenched flow reactor.ONOO~-at 1 mmol/L concentration was added to cultured osteoblast system in vitro.After incubated for 30 min,osteoblasts were continued to be cultured for 12 h with routine method.Hoechst33258 stain and TUNEL method were used to detect the osteoblast apoptosis and 0.1 mmol/L concentration of paeonol was used to eliminate the effect of ONOO~-(1 mmol/L) on osteoblast apoptosis of rat.Results ONOO~-(1 mmol/L) could lead to rat osteoblast apoptosis.Paeonol of 0.1 mmol/L concentration could antagonize rat osteoblast apoptosis induced by ONOO~-(1 mmol/L).Conclusion Paeonol(0.1 mmol/L) has the significant antagonizing effect on ONOO~-of rat osteoblast apoptosis in vitro.
