Fingerprints of Rheum tanguticum from Qinghai Region by HPLC
- VernacularTitle:青海地区唐古特大黄药材HPLC指纹图谱研究
- Author:
Jinping LI
- Publication Type:Journal Article
- Keywords:
Qinghai;
Rheum tanguticum Maxim.ex Balf.;
HPLC;
fingerprints
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC fingerprints of Rheum tanguticum from Qinghai Region.Methods By RP-HPLC,acetonitrile and water including 0.04% H_3PO_4 as mobile phase,gradient elution,flow rate was 1.0 mL/min,the detection wavelength was 280 nm,and the temperature was 40 ℃.Results The RSD of peak areas and retention time of common peak in the test of precision,repeatability,and stability has not reached 5%,respectively.Average similarity was 0.925 in R.tanguticum collected from different habitats.Conclusion All above results show that this method is simple,practicable,and reliable.It can be used as a standard analysis method in the quality control of R.tanguticum from Guoluo Region in Qinghai Province.It also provides a theory foundation on which the species cultivated could replace the wild ones.
