Silence the expression of c-erbB-2 and Bcl-2 gene in Tca8113 cell line by rnai technique
- VernacularTitle:表达重组干扰质粒对Tca8113细胞c-erbB-2、Bcl-2基因表达干扰作用的实验研究
- Author:
Ping JI
- Publication Type:Journal Article
- Keywords:
Recombined interfering plasmid;
Liposomes transfection;
Gene expression;
Fluorescence-quantitative RT-PCR
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective: To investigate the interfering efficiency of RNAi technique on the expression of c-erbB-2 and Bcl-2 oncogene in human tongue squamous carcinoma(Tca8113).Methods: The recombined RNAi plasmids for c-erbB-2 and Bcl-2 oncogene were constituted by the four succussive steps-designing of Oligo DNAs,synthesis of Oligo DNAs,transfection of Oligo DNAs into pSUPER.neo+gfp vectors and selection of positive plasmids.In order to silence the expression of c-erbB-2 and Bcl-2 oncogenes,the recombined RNAi plasmids were transfected into Tca8113 cells by culturing together for about 10 hours,and the interfering efficiency of RNAi for the two oncogenes was evaluated by fluorescence-quantitative RT-PCR.Results: The interfering efficiencies for c-erbB-2 oncogene were 62.68% and 40.61%,respectively in psiC1?psiC2?psiC3,while the interfering efficiencies for Bcl-2 oncogene were 66.20% and respectively in psiB1?siB2?psiB3.Conclusion: The recombined RNAi plasmids of psiC2(gAgTCCCAACCATgTCAAA) for c-erbB-2 oncogene and of psiB2(CCgggAgATAgTgATgAA) for Bcl-2 oncogene can effectively silence the expression of c-erbB-2 and Bcl-2 oncogenes in Tca8113 cell.
