PEGylation of adenovirus vectors and its features
- VernacularTitle:腺病毒载体的PEG修饰及其特性研究
- Author:
Feng QIU
- Publication Type:Journal Article
- Keywords:
Adenovirus vector;
Polyethylene glycol
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To enhanced the physical stability of adenovirus vectors(Adv) and ablated humoral immune responses by PEGylation of adenovirus with polyethylene glycols.Methods: PEGylated Adv(PEG-Adv) was constructed by PEGylated adenoviral capsid proteins with activated methoxypolyethylene glycols,and gene expression was evaluated using A549 cells.Furthermore,the plasma half life and the antibody evasion ability were also evaluated.Results: PEG-adv could be protected from antibody neutralization in the high antibody titers to adenovirus,suggesting that PEGylation improve of the ability to administer Adv on a repeated basis.Conclusion: PEGylated Adv can maintain strong protective activity against antibodies.The approach described here could form the basis for further development of adenoviral gene therapy vectors with improved pharmacokinetics and increased efficiency of therapeutic gene transfer in disease.
