The study of green tea polyphenols inhibiting apoptosis of lens epithelial cell in vitro and relevant mechanism
- VernacularTitle:茶多酚抗晶状体上皮细胞凋亡的体外研究及机制
- Author:
Xi ZHOU
- Publication Type:Journal Article
- Keywords:
Green Tea Polyphenols;
Lens epithelial cell;
Oxidative injury;
Apoptosis;
Mechanism
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect of Green Tea Polyphenols(GTP) on H2O2-induced lens epithelial cell apoptosis and relevant mechanism.Methods: Separated and cultivated lens epithelial cell,disposed that with GTP,then detected the inhibitory effect of GTP by electron transmission microscopy,flow cytometry and DNA fragmentation assay.The bcl-2,Bax and caspase-3 protein expression were measured with Western-blot in various groups.Results: The apoptosis cells and G1 phase cells increased in the group treated with sodium chloride solution,and the DNA "ladder" was found.In the group treated with GTP,the apoptosis cells and G1 phase cells decreased,and no DNA "ladder".The apoptosis protein bcl-2 increased,however,the Bax and caspase-3 protein decreased.Conclusion: The GTP can protect lens epithelial cells from the oxidative injury and restrain the apoptosis rate of lens epithelial cells and the mechanism was nearly correlated with bcl-2,Bax and caspase-3 protein.
