Establishment of human leukemia model by transplanting K562 cells into SCID mice
- VernacularTitle:移植性人白血病小鼠模型的建立
- Author:
Dilong CHEN
- Publication Type:Journal Article
- Keywords:
K562 cells;
Severe combined immunodeficiency mice;
Animal model
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the transplanted leukemia severe combined immunodeficiency(SCID) mouse model and to explore the biological action of human leukemic cells in SCID mice.Methods: 5?106～1?107 K562 cells were inoculated into the abdominal cavity or vein of SCID mice.Immunocytochemistry,RT-PCR,flow cytometry and histopathology were used to detect the leukemic cells in the peripheral blood,bone marrow,liver and spleen of SCID mice.Results: K562 cells could be transplanted into SCID mice through abdominal cavity or vein,and leukemia animal model was successfully established.3 weeks after transplantation,the leukemia cells could be found in the peripheral blood,The proliferation phase cells in the liver and spleen reached 40.11% and 41.66% respectively.In the terminal stage,the CD13+ cells accounted for 10.15% of bone marrow cells in model animal.The DQ?and ABL-BCR genes could be detected after injection of K562 cells,and 3 to 4 weeks after injection,the leukemic cells spread to the liver,spleen,kidney,bone marrow,and so on.Conclusion: The leukemia animal model could be successfully established by transplanting K562 cells into SCID mice.Positive rate of CD13 might be used as the index of tumor cells infiltration.
