Expression of AQP1 in ocular tissues of rat
- VernacularTitle:大鼠眼内组织水通道蛋白1的表达
- Author:
Jiawen LI
- Publication Type:Journal Article
- Keywords:
Aquaporin-1;
Immunohistochemistry;
Ocular tissues
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of aquaporin-1(AQP1) in ocular tissues of normal rats.Methods:The eyeballs were enucleated from the male Wistar rats,fixed with 4% formaldehydum polymerisatum and embeded in paraffin.Then,5?m serial section was made on the sagittal plane.Each sample was cut into 3 pieces for AQP1 HE staining and immunohistochemical staining,the latter of which included negative control and positive control.The immunohistochemical staining of renal tubule,known as positive expression of AQP1,was used as positive control.Results:Under the light microscope,the brown cellular granules indicated positive-AQP1 cells.AQP1 was expressed in corneal endothelial cells,iris epithelial cells,crystalline lens epithelial cells,ciliary body non-pigment epithelial(NPE) cells,ganglion cell layer and inner nuclear layer of retina,optic nerve glial cells.Conclusions: AQP1 is expressed in many places of ocular tissues related to the water metabolism.
