Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor
- VernacularTitle:人羊膜上皮细胞在纤维蛋白支架和细胞生长因子作用下的研究
- Author:
Wei LI
- Publication Type:Journal Article
- Keywords:
HAEC;
fibrin clot;
growth factor
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the model of cultured human amniotic epithelial cells(HAECs) on the fibrin clot in order to observe the effect of EGF,bFGF and TGF-?1 on HAEC.Methods:The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy,Giemsa staining and scanning electron microscopy.The effect of growth factor(EGF,bFGF,and TGF-?1) on the apoptosis of HAECs was measured by TUNEL.Results: HAECs generated on the formulated fibrin clot in vitro.HAECs exposed to EGF or bFGF showed a lower rate of apoptosis than the control group(P